摘要
采用壳聚糖固定TCNQ和生物酶在泡沫镍载体表面制备成电化学生物传感器电极，用循环伏安法研究了该胆碱酯酶电极的电化学性质．结果表明：泡沫镍因其良好的导电性有助于降低氧化电位，当饱和TCNQ丙酮溶液与壳聚糖溶液混合比例为1：1．5和壳聚糖溶液与酶液比为1：1时，胆碱酯酶电极性能最好．胆碱酯酶电极的抑制百分率与毒死蜱农药浓度的对数lgC在1．0×10^-4～1．0×10^-9mol／L范围内均呈现良好的线性关系，其检测限可达到6．95×10^-11mol／L．经过多次重复试验表明该酶电极具有良好的重复稳定性．
Electrochemical biosensor electrodes was fixed with TCNQ and enzyme on the surface of nickel foam carrier by using chitosan, Cyclic voltammetry is used to study the electro-chemical properties of cholinesterase electrodes. The results showed that the high conductivity of nickel foamy reduces the oxidation potential. When saturated TCNQ acetone solution with chitosan solution mixing ratio of 1 ： 1. 5 and the chitosan solution and enzyme liquid ratio of 1 ： 1, Cholinesterase electrode performance is best. The inhibition of chlorpyrifos was proportional to its concentration ranging from 1.0×10^-4～1.0×10^-9mol/L with a detection limit 6.95×10^-11mol/L. The developed Cholinesterase electrode exhibited good reproducibility and acceptable stability.
出处
《浙江工业大学学报》
CAS
2014年第2期137-141,共5页
Journal of Zhejiang University of Technology
基金
浙江省自然科学基金资助项目(Z13H040356)
"新材料及加工工程"浙江省重中之重学科开放基金资助项目(20110928)
关键词
胆碱酯酶
泡沫镍
TCNQ
壳聚糖
毒死蜱
aeetylcholinesterase
nickel foam
TCNQ
chitosan
chlorpyrifos
