初探日本血吸虫SWA及SEA对Ⅰ型糖尿病小鼠血糖的抑制作用

Preliminary Japonicum SWA and SEA MiceⅠ Diabetes Glucose Inhibition

目的:探讨日本血吸虫SWA及SEA对实验性Ⅰ型糖尿病小鼠血糖的抑制作用。方法:制备日本血吸虫可溶性成虫抗原(Soluble worm antigen,SWA)与可溶性虫卵抗原(Soluble egg antigen,SEA)并构造实验性Ⅰ型糖尿病小鼠模型。把24只糖尿病实验小鼠随机平分为A、B、C组(每组8只)。A组实验小鼠从腹部皮下多点免疫注射日本血吸虫SWA;B组实验小鼠从腹部皮下多点免疫注射日本血吸虫SEA;C组实验小鼠用PBS进行腹部皮下多点免疫,3组小鼠每周免疫1次,连续免疫4周后,采小鼠尾静脉血,检测实验小鼠的血糖变化。结果:B组实验小鼠的血糖在4周左右为(9.85±1.17)mmol/L,有明显降低,相比C组小鼠血糖(12.14±1.25)mmol/L具有显著性差异(P<0.01),但A组实验小鼠血糖在4周左右为(12.11±0.67)mmol/L与C组小鼠血糖无显著差异(P>0.05)。结论:日本血吸虫SEA对实验性Ⅰ型糖尿病小鼠的血糖具有一定的抑制作用,其机制可能是日本血吸虫SEA刺激了机体的免疫应答,导致Th1反应向Th2反应偏移。

To explore the inhibited effect of Japan Schistosoma SWA and SEA on experimental type diabe-tes mellitus mice .Methods ：Constructed type diabetes mellitus mice model and prepared Japan soluble worm soluble a-dult Antigen of Schistosoma antigen ,SWA and soluble egg Antigen in soluble egg antigen ,SEA for experiment .The modeling 24 mice were randomly divided into 3 groups named A ,B and C ,and 8 mice in each group .Group A in diabet-ic model rats immunized by subcutaneous injection Japan SWA Schistosoma ;group B diabetes model rats immunized by subcutaneous injection Japan SEA the schistosomiasis ;group C Antigen replaced by PBS in diabetic model rats abdomi-nal subcutaneous immunization ,immunizated once a week ,after 4 times ,collected tail vein of mice blood ,tested blood glucose in mice .Results：Group B blood glucose in mice started 3 weeks was slightly lower ,about 4 weeks fell （9 .85 ± 1 .17）mmol/L ,significantly lower than group C （12 .14 ± 1 .25）mmol/L ,the difference was significant （P〈0 .01） , but the mice （12 .11 ± 0 .67）mmol/L in group A and C groups were not significantly different （P〉0 .05） .Conclusion：Japan SEA of schistosomiasis on blood sugar of mice with experimental type diabetes mellitus have some lowering effect which may stimulate the body’s immune response ,causes Th1/Th2 immune deviation .