冰糖橙×枸橼杂交后代的胚抢救及杂种鉴定

Embryo rescue and hybrid identification of sweet orange(Bingtang) and citron

为获得冰糖橙×枸橼的合子苗,以抗柑橘溃疡病枸橼为父本,以高感病品种冰糖橙为母本进行杂交,利用胚抢救技术获得杂交后代,然后利用筛选出的SSR引物对亲本和子代的基因组DNA进行PCR扩增,分析并鉴定杂交后代中的合子苗。结果表明:在最终获得的359株杂交后代中,利用SSR标记能鉴定出合子苗12株。

In order to obtain zygotic seedlings from hybrid of sweet orange （Bingtang） and citron, a experiment was conducted by taken Bingtang as female parent and citron with resistance to canker as male parent. Embryo rescue technology was firstly used to obtain filial generation, and then the genomic DNA of parents and their hybrids were amplified by PCR using screened simple sequence repeats （SSR） primers, and zygotic seedlings of next generation were further identified. The results showed that twelve zygotic seedlings were identified from the 359 obtained seedlings by SSR markers.