金边龙舌兰超氧化物岐化酶的提取与特性研究

Study on extraction and properties of SOD from Agave americana variegata marginata hort leaf

金边龙舌兰经匀浆、丙酮沉淀、葡聚糖凝胶过滤等步骤,纯化获得2个超氧化物歧化酶（ SOD） I和Ⅱ。该SODI和Ⅱ适应pH范围分别在4-11和4-10,pH分别为7和8时活性最高；均在60℃以下稳定,60℃能够保持较高的活力；分别在272．5和274．5 nm处有最大紫外光吸收；敏感性抑制试验证明,其活性均被氰化钾和过氧化氢所抑制,但不受氯仿-乙醇影响,与Cu·Zn-SOD相似。

Superoxide dismutases（ SOD）Ⅰ andⅡwere purified from Agave americana variegata marginata Hort,which was homog-enate,precipitation by acetone and gel filtration on Sephadex G column. The enzymes were relatively stable at temperatures below 60℃. At 60℃,the enzymes had the most activity. At pH ranging from 4-10 and 4-11,the enzymes were relatively stable. At pH 7 and 8,the enzymes had the most activity,respectively. They had the absorption maximums of ultraviolet absorption spectroscopy at 272. 5 and 274. 5 nm,respectively. They were inhibited by potassium cyanide and hydrogen peroxide,but not influcend by CHCl3-C2 H5 OH,which was similar to copper-and zinc containing SOD.