水稻黑条矮缩病毒P9-1在烟草表皮细胞中自我互作并形成包涵体

Rice black streaked dwarf virus P9-1,self-interacts and forms inclusion bodies in epidermal cells of Nicotiana benthamiana plants

水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV)是斐济病毒属的成员,由昆虫介体灰飞虱传播,能够在植物宿主和昆虫介体中复制。同其他斐济病毒成员一样,RBSDV的复制与装配是在细胞质病毒包涵体结构即毒质结构里进行的。RBSDV有10条基因组dsRNAs(S1—S10),其第9条片段(S9)的第一个阅读框(ORF)编码的蛋白P9-1是形成毒质结构的框架蛋白。将P9-1与绿色荧光蛋白(GFP)融合后,在本氏烟草的表皮细胞中单独表达,通过激光共聚焦扫描显微镜观察发现P9-1可以形成包涵体结构;利用双分子荧光互补(BiFC)实验证明了P9-1具有自我互作的能力,并形成包涵体结构。

Rice black-streaked dwarf virus （RBSDV）, a member of the genus Fijivirus, is transmitted in nature by the small brown planthopper （Laodelphax striatellus） and can replicate in cells of both host plants and insect vector . RBSDV, like other members of the family Reovirus , is thought to replicate and assemble within cytoplasmic viral inclusion bodies, commonly called viroplasms.It has genomic segments of 10 dsRNAs （S1-S10） and P9-1 encoded by the first open reading frame in the ninth segment （ S9） of the genomic RNAs is an essential protein for the formation of viroplasms.Fused with green fluorescent protein （GFP）, P9-1 was able to form inclusion bodies when expressed alone in Nicotiana benthamiana epidermal cell.Bimolecular fluorescence complementation （BiFC） assay suggested P9-1 had the ability of self-interaction and formed inclusion bodies .