摘要
以改进的CTAB法对何首乌总基因组DNA进行提取,采用通用引物对不同来源的何首乌rDNA ITS序列进行PCR扩增、测序和序列分析。结果表明,何首乌rDNA完全序列片段长度共约652 bp,其中ITS1的长度为202 bp,5.8 S的长度为161 bp,ITS2长度为232 bp,与其近缘种ITS序列间存在明显差异。其rDNA ITS序列在分子水平上为鉴别何首乌提供了参考依据。
With improved CTAB meth different sources of results showed that Polygonum rDNA Polygonum rDNA od, Polygonum total genomic DNA was extracted ITS sequences for PCR amplification, sequencing sequence is a 652 bp fragment, in which the are 202 bp, 161 bp, and 232 bp, respectively. The ITS sequences indicate difference from its related species . The rDNA ITS sequences at the molecular leve identification of Polygonum. using universal primers for and sequence analysis. The lengths of ITS1, 5.8 S and ITS2 that Polygonum has significant 1 can provide a reference for the
出处
《生物信息学》
2014年第1期27-32,共6页
Chinese Journal of Bioinformatics
关键词
何首乌
ITS序列
PCR
Polygonum muhiflorum
ITS sequence
PCR
