大鼠慢性胃炎模型的快速建立及致萎缩因素探讨

The Establishment of Experimental Model of Chronic Gastritis in Rats and Factors Inducing Atrophic Gastritis

目的 :建立快速鼠慢性胃炎模型 ,并探讨所用的刺激因素对胃粘膜致萎缩的作用。方法 :采用正交设计 ,综合运用 60 %酒精、2 0 mmol/L去氧胆酸钠和 0 .0 5 %氨水刺激 SD大鼠胃粘膜 ,并应用吲哚美辛及低维生素类食物喂饲大鼠 ,1 2周后观察鼠胃粘膜的病理改变。结果 :综合法刺激 1 2周后鼠胃粘膜炎症程度有显著变化 ,胃窦固有腺数明显减少 ,胃体壁细胞数减少 ( P<0 .0 1 ) ;吲哚美辛只引起鼠胃粘膜炎症 ,无萎缩变化。短期低维生素食物无致炎症及萎缩作用 ,且后两者对综合刺激无协同作用。结论 :综合法能建立快速大鼠慢性胃炎模型 。

Objective:To establish experimental animal model of chronic gastritis and study the related role of inducing factors on atrophic gastritis.Methods:Three treatments were used to induce chronic gastritis in rats:①combined intragastric administration with 60% alcohol,20 mmol/L sodium deoxycholic acid and 0.05% ammonia;②giving forage containing low vitamins;③intragastric administration with indomethacin.A L 8(2 7) orthogonal design was adopted in experiments.After 12 weeks,pathological examinations of gastric mucous were done.Results:The inflammation grades of rats with treatment①were statistically different with those of the control,the number of pyloric glands at antrum and number of parietal cells at body being markedly reduced( P <0.01);Indomethacin only induced gastric inflammation but not atrophy;short term vitamin deficiency forage did not induce gastritis and gastric atrophy.In addition,indomethacin and vitamin deficiency didn't exacerbate the effect of treatment①.Conclusion:Combined administration could induce short term establishment of animal model of chronic gastritis and induce atro phic gastritis.