百日草组织培养技术的研究

Research on tissue culture technique of Zinnia elegans

以百日草茎段为外植体,对其进行组织培养技术研究,结果表明:不同生长阶段的茎段对相同的消毒处理产生不同的反映,以生长中等的茎段诱导腋芽萌发的效果最为理想,适宜的灭菌方法为70%酒精处理20 s、再用0.1%HgCl2处理8+4 min。最合适的腋芽诱导培养基为MS+KT0.2 mg·L-1+BA2.0 mg·L-1+GA0.5 mg·L-1,外植体污染率较低,诱导率较高;试管苗继代增殖效果最佳的培养基为MS+BA1.0 mg·L-1,不仅增殖系数高,达到8.67,而且试管苗生长健壮;生根效果最好的培养基为MS+NAA0.1 mg·L-1,试管苗根系生长粗壮,生根数量多,平均根长较短。

Stems of Zinnia elegans were taken as explants to research on the tissue culture technique; the results showed that the responses of different growth stages of stems for the same disinfection treatment were different .Medium stem had the most ideal effect of axillary bud sprouting , the suitable sterilization method was with 70%alcohol for 20 s, and then re-used with 0.1%HgCl for 8＋4 minutes.MS＋KT0.2 mg· L-1 ＋BA2.0 mg· L-1 ＋GA0.5 mg·L-1 was the most appro-priate medium of axillary bud induction , with low pollution rate of explant and high induction rate;MS＋BA1.0 mg·L-1 was the most appropriate medium , which had the best multiplication effect of subculture of test -tube plantlet ;growth co-efficient reached 8.67, and the test-tube plantlet grew strongly;MS＋NAA0.1 mg mg· L-1 was the most appropriate me-dium of rooting effect , the roots of test-tube plantlet grew strongly , the average root length was shorter , and quantity was large.