单克隆免疫亲和柱-高效液相色谱法测定酱油中伏马毒素B_1、B_2

研究了单克隆免疫亲和柱 -高效液相色谱法测定酱油中伏马毒素B1、B2 的方法。样品经离心和玻璃纤维滤纸过滤后 ,通过FumoniTest免疫亲和柱净化 ,净化液经柱前衍生 ,SpherisorbC18色谱柱分离 ,荧光检测器检测 ,外标法定量。对添加不同含量水平的伏马毒素B1、B2 ,5次重复实验的平均回收率为B184 .6 % - 89.2 % ,B2 6 0 .3% - 6 9.5%。变异系数 (CV)为B15.5%～ 7.8% ,B2 6 .2 %～ 9.3%。检测低限为B10 .0 1mg/kg ,B2 0 .0

A method for determination of fumonisins B 1 and B 2 in soy sauce by High Performance Liquid Chromatography is reported.The sample is cleaned up by FumoniTest affinity cartridges. Fluorescent fumonisins derivatives are formed by adding OPA and are separated on Spherisorb C 18 column ,and are detected by fluorescent detector and are quantified by external standard. Soy sauce samples are fortified with a misture of fumonisins B 1 and B 2 at the four levels.Five replicate analysis are performed each level .The recoveries of the fumonisins are 84.6%-89.2% for B 1 and 60.3%-69.5%for B 2.The variation coefficients are 5.5%～7.8% for B 1 and 6.2%～9.3%for B 2.The detecttion limits are 0.01mg/kg for B 1 and 0.02mg/kg for B 2.