摘要
目的观察地骨皮醇提液对脂多糖(LPS)诱导的大鼠肾脏系膜细胞(HBZY-1)单核细胞趋化因子(MCP-1)表达的影响。方法常规培养HBZY-1细胞,将细胞分为正常对照组、单独LPS作用组、药物预处理(低、中、高浓度)+LPS作用组。ELISA法测定细胞上清MCP-1含量,RT-PCR法检测细胞因子MCP-1mRNA表达。结果 LPS能够显著提高HBZY-1细胞MCP-1释放量(P<0.01),地骨皮醇提液预处理后,MCP-1含量降低(P<0.05,P<0.01)。LPS能明显激活MCP-1mRNA表达(P<0.01),地骨皮醇提液预处理过后,能明显降低MCP-1mRNA表达(P<0.05,P<0.01)。结论地骨皮醇提液能够抑制LPS诱导的大鼠肾脏系膜细胞(HBZY-1)MCP-1mRNA的表达。
Objective To investigate the effect of ethanol extract of Cortex Lycii on monocyte chemotactic pro-tein 1 （MCP-1）expression in lipopolysaccharide（LPS）-induced murine renal mesangial cells（HBZY-1）. Methods HBZY-1 cells were conventionally cultured and divided into three groups： normal cell group, LPS-treated alone group, and Cortex Lycii＋LPS-treated group. In a high-glucose condition, HBZY-1 cells were preincubated with dif-ferent concentrations（0.2 ,0.4, and 0.8 mg/mL）of Cortex Lycii for 4h and then LPS of 10 μg/mL was added into 3 Cortex Lycii groups and LPS-treated alone group for another 24h. The release amount of MCP-1 in supernatant was detected by ELISA. The expression of MCP-1 mRNA was determined by RT-PCR. Results LPS markedly in-creased the release amount of MCP-1 in supernatant of HBZY-1 cells, but after treated with Cortex Lycii, the amount of MCP-1 decreased（P〈0.01）. LPS evidently activated the expression of the MCP-1 mRNA in HBZY-1 cells （P〈0.01）, but the pretreatment with Cortex Lycii decreased the expressions of MCP-1 mRNA（P〈0.05, P〈0.01）. Conclusion Cortex Lycii can inhibit the expression of MCP-1 mRNA in HBZY-1 cells induced by LPS.
出处
《浙江中西医结合杂志》
2014年第4期293-295,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
