围刺配合电针对乳腺增生大鼠血清激素水平和乳腺组织形态、ER表达的影响

The effect of Weici combined with electric acupuncture on the expression levels of E2 /P ,prolactin,testosterone,ERand mammary gland tissue morphous in rats with mammary gland hyperplasia

目的观察围刺配合电针对乳腺增生大鼠血清性激素雌与孕激素的比值(E2/P)、泌乳素(PRL)、睾酮(T)、乳腺组织形态和乳腺组织ER表达的影响,以探讨干预治疗乳腺增生的作用机制。方法随机将40只Wistar大鼠分为模型组、针刺组、三苯氧胺组及正常组,每组10只。采用己烯雌酚联合黄体酮肌内注射进行造模。针刺组:将大鼠固定,分别围刺第2对左右乳房后,接通电针仪持续刺激30 min,并针刺膻中穴,留针30 min;三苯氧胺组予三苯氧胺1.8 mg/kg灌胃治疗,1次/d,共30 d。于末次治疗后测定血清E2、P、PRL、T含量,计算E2/P比值;取大鼠第2对乳房常规HE染色,光镜下观察组织形态表现;运用免疫组化法对乳房乳腺组织中ER表达情况进行观察。结果治疗后E2/P:针刺组与三苯氧胺组比值均低于模型组(P<0.05)。PRL:针刺组和三苯氧胺组均低于模型组(P<0.05);针刺组与三苯氧胺组比较差异有统计学意义(P<0.05)。T:针刺组和三苯氧胺组均低于模型组(P<0.05)。乳腺组织ER表达:针刺组和三苯氧胺组大鼠乳腺组织ER阳性细胞表达情况显著降低,AOD值低于正常组(P<0.05)。结论围刺配合电针具有降低模型大鼠乳头高度、缩小乳头直径,改善血清E2/P、PRL、T紊乱状态和乳腺组织病理形态,降低乳腺增生模型大鼠乳腺组织ER表达的作用。

Objective To observe the effect of Weici combined with electric acupuncture on E2/P,prolactin（PRL）, testosterone（T）, ER expression and mammary gland tissue morphous in rats with mammary gland hyperplasia in order to explore its action mechanism. Methods The 40 Wistar rats were randomly divided into 4 groups ： normal control group, model group,acupuncture group and tamoxifen （TAM） group. The animal models with breast hyperplasia were established by intramuscular injection with diethylstilbestrol ＋ progesteroner. The rats in acupuncture group were fixed and acupunctured （Weici） in the second pair of breasts, and connected with the electric acupuncture instrument for 30 min, moreover, Danzhong acupuncture point was acupunctured for 30min;the rats in TAM group were given tamoxifen 1.8mg/kg by garage, once a day for 30 days, after last treatment, the serum levels of E2, P, PRL, T were detected, and the ratio of E2/P was calculated, the second pair of breasts were taken to be HE stained, then the changes of breast tissue morphous were observed under light microscope, and the expression levels of ER in breast tissue were detected by immunohistochemisty. Results After treatment, the ratio of E2/P in acupuncture group and TAM group was significantly lower than that in model group （ P 〈 0.05 ） ; the serum levels of PRL in acupuncture group and TAM group were obviously lower than those in model group （ P 〈 0.05 ）, moreover, there were significant differences between acupuncture group and TAM group （ P 〈 0.05）. The serum levels of T in acupuncture group and TAM group were significantly lower than those in model group （ P 〈 0.05）. The pathological changes of breast tissue in acupuncture group were similar to those in TAM group, however, which were obviously improved, as compared with those in model group. The expression levels of ER of breast tissue in TAM group were significantly decreased, furthermore,the AOD value was obviously lower than that of normal control group （ P 〈 0.05）. Conclusion Weici combined with electric acupuncture can decrease nipple height and nipple diameter, improve disorder state of E2/P, PRL, T and pathological morphous of breast tissue, and reduce ER expression levels in breast tissue of rats with breast hyperplasia.