串珠镰刀菌诱导玉米活性氧及保护酶系变化研究

Changes of Active Oxygen and Protective Enzymes in Maize induced by Fusarium moniliforme

以不同抗病品种(BT-1)、感病品种(N6)的玉米幼苗为材料,研究了在串珠镰刀菌诱导下玉米幼苗POD、SOD活性、O2·-产生速率以及MDA的含量变化。结果表明,接种后O2·-产生速率和MDA的含量增大,抗病品种均高于感病品种,抗病品种的O2·-产生的速率峰值出现早且高,接种36 h达到高峰,且是对照的5.8倍;抗病品种SOD酶活性变化明显,且高于感病品种;接种后POD活性增加,感病品种活性增幅高,高于抗病品种。说明抗感玉米品种接种串珠镰刀菌后,相关酶活性的变化与活性氧的产生存在着明显的差异,这种变化在一定程度上反映了抗感玉米植株在病原菌胁迫下其不同的调节机制。

Using resistant maize＇ BT- 1＇ and sensitive maize＇ N6＇ as materials, O2·- production rate, MDA content, POD and SOD activity in maize seedlings were measured after induced by Fusarium moniliforme. The results showed thatO2·- production rate and MDA content increased after inoculation, and they were higher in resistant cuhivars higher in sensitive cultivars. The peak of O2·- production rate in resistant cultivars appeared early and high, which reached its maximum at 36 h, being 5.8 times of control. Changes of SOD activity were apparent in resistant eultivars and higher than sensitive cuhivars. POD activity was increased after inoculation, but higher in sensitive cultivars than resistant cultivars. It was suggested that protective enzymes and active oxygen appeared apparent differences in resistant and sensitive cultivars after inoculation by Fusarium moniliforme, the changes may reflect the different regulatory mechanisms in resistant and sensitive maize induced by pathogen.