间接原子吸收法定量测定菜籽粕中植酸的研究

Indirect AAS Determination of Phytic Acid in Rapeseed Meal

研究了间接原子吸收定量测定菜籽粕中植酸含量的方法。采用三氯乙酸提取植酸 ,添加三氯化铁 ,使其生成植酸铁沉淀 ,用原子吸收法测定上清液中剩余铁的含量 ,推算植酸沉淀铁的量 ,以定磷法得出的植酸含量为比较基准 ,结果表明植酸与铁离子 (Fe3+)以 6.0∶1的比例络合 ,根据这一比例关系 ,测出试样中植酸沉淀Fe3+的含量 ,即可求算样品中植酸的含量。

The experiment showed that isolating phytic acid from rapeseed meal and reacting the phytic acid with FeCl 3,the phytic acid combined the Fe 3+ with a ratio of 6∶1 (mol/mol).So the phytic acid in a rapeseed meal sample can be measured indirectly by detemining the Fe 3+ combined with the phytic acid in the reaction.