新疆昌吉地区部分规模化猪场PRRSV抗体监测和免疫效果分析

Identification of Swine PRRSV Antibody and Immunization in Some Intensive Large-Scale Pig Farms in Changeji Region

为了查明昌吉地区地区部分规模化猪场猪PRRSV的抗体阳性率及免疫合格率,分别采集了昌吉地区4个免疫了弱毒苗的规模化猪场,和3个免疫了灭活苗的规模化猪场血清共1 036份,采用间接ELISA方法检测其抗体.经检测,4个免疫PRRS弱毒苗规模化猪场总阳性率为79.05％（468/592）,平均KQ为49.63,标准差为39.41.3个免疫PRRS灭活苗规模化猪场总阳性率为62.61％ （278/444）,平均KQ为45.88,标准差为31.22.结果表明,在规模化养猪场使用灭活苗免疫预防PRRS感染作用不如弱毒苗,因此,建议临床应用中应选择弱毒苗为宜,结合检测结果、临床生产数据及猪场已采用猪PRRS免疫程序,建议商品猪在仔猪断奶后初免.在高致病性猪蓝耳病流行地区,可根据实际情况在初免后1个月加强免疫一次,种母猪70日龄前免疫程序同商品猪,以后每次配种前加强免疫一次,种公猪70日龄前免疫程序同商品猪,以后每隔4～6个月加强免疫一次.

To identify rates of positive PRRSV antibody and immunization qualified ratio, 4 pig farms inoculated with atten- uated vaccine and 3 farms inoculated with inactivated vaccine have been examined. 1036 serum samples were tested for antibody using indirect ELISA. The results showed that rate of positive antibody of the 4 farms was 79.05~ （468/592） and average KQ was 49.63. Rate of positive antibody in the 3 farms was 62.61M （278/444） and average KQ was 45.88~ Therefore, attenua- ted vaccine was more effective for PRRSV than inactivated vaccine in large--scale pig farms, attenuated vaccine is recommended for PRRSV control in intensive large-scale pig farms. To apply the results of this study on pig reproduction and vaccination procedure in intensive farms, we also suggest that a booster immunization be given 1 month after the first vaccination in HPAIV areas. Vaccination on breeding sows before 70--day--old has same procedure as commercial pigs, boosting vaccina- tions before mating are recommended. Vaccination on breeding boars before 70--day--old has same procedure as commercial pigs, boosting vaccinations in every 4--6 months are recommended.