聚乳酸-壳聚糖软骨支架的性能测定

Determination properties of poly-L-lactic acid-g-hydroxyethyl chitosan composite scaffold

目的制备左旋聚乳酸-g-羟乙基壳聚糖(PLLA-g-HECS)复合支架,并进行性能测定。方法采用热致相分离法制备PLLA-g-HECS聚合物,再用压片机,将聚合物压模成形,测定复合支架的微观形貌、X射线衍射图谱分析、溶解实验及溶血试验。结果复合支架具有纳米微米共存的高孔隙直径的亚微观结构。X射线衍射图谱说明引入左旋聚乳酸链段后进一步减弱了HECS分子间和分子内较强的氢键作用。结论复合支架溶解实验显示复合物有较好的溶解性。溶血实验提示复合支架复合支架不会引起急性溶血反应,具有良好的生物相容性。

Objective To fabricate the poly-L-lactic acid-g-hydroxyethyl chitosan （PLLA-g-HECS） composite scaffold and determinate properties. Methods The PLLA-g-HECS was prepared by thermally induced phase separation method,and then used the tableting machine to compress composite scaffolds,and determinate X-ray diffraction pattern analysis,dissolution experiments and hemolysis test of composite scaffolds. Results The composite scaffolds showed a porosity submicroscopic structure combined nanometer,micrometer structure. X-ray diffraction patterns showed that the introduction of L-polylactic acid segment further weak-ened the hydrogen bonds between HECS and intramolecular. Conclusion Dissolution experiments of composite scaffolds show better solubility,hemolysis experiments suggest scaffold scaffold does not cause acute hemolytic reaction,has good biocompatibility.