Asp.niger No.3液态发酵生产单宁酶条件的研究

Study on Optimal Conditions to Produce Tannase by Fermentation of Asp. Niger No.3

研究不同碳源、氮源种类和比例及不同起始pH值等因素对黑曲霉生产单宁酶的影响；并考察了豆粕+NH4NO3分别与单宁、K2HPO4的交互作用。结果表明：不同碳源、氮源种类和比例、不同起始pH值等因素对黑曲霉生产单宁酶的影响较大，单宁1.25，小麦粉0.35，豆粕0.67%，NH4NO3 0.05%，K2HPO4 0.035，pH6.0为产酶的适宜条件，最高酶活达到144. 25U/100ml发酵液，酶比活为17.71。豆粕+NH4NO3、单宁、小麦粉对产酶有极显著影响；豆粕+NH4NO3与K2HPO4的交互作用极显著，与单宁的交互作用显著。本文还探索了菌丝生长与产酶的关系，结果表明：黑曲霉产单宁酶属于生长偶联型。

The effects of different factors on producing tannase have been studied,such as different carbon sources ,carbon source ratio,different nitrogen sources,nitrogen source ratio and initial pH value.The optimal mixture and the optimal culture conditions of liquid state fermentation to produce tannase have been obtained by making orthogonal tests.The results showed that the optimal mixture was soyabean meal (0.67%),tannin acid (1.2%) wheat flour (0.3%),NH4NO3(0.05%) ,KCl (0.05%),MgSO47H2O (0.05%),FeSO4 (0.001%),K2HPO4 (0.03%),and pH6.0.The tannin acid,the combination of soyabean meal and NH4NO3,and wheat flour have shown great effects on producing tannase.Moreover,the combination of soyabean meal and NH4NO3 has shown positive interaction with tannin acid,and the combination of soyabean meal and NH4NO3 has shown also very notable positive interaction with K2HPO4.pH6.0 of the mixture was benefit to producing tannase.The investigation on the strain’s growth and the time of producing tannase showed that the biomass,protein content and the yield of tannase reached maximum in 72 hours.Therefore,it could be concluded that the strain’s capacity to produce tannase was parallel to its growth.