多管藻(P.urceolata)复合氨基酸营养液的研制

Study on Preparation of Nutritive Liquid of Composite Amino Acids from Enzymatic Hydrolysis of P.urceolata

本研究就多管藻（Polysiphonia urceolata）的脱色方法及酶解工艺条件等进行了探讨。结果表明原料脱色用KMnO4效果较好，酶解的最佳工艺条件是使用中性蛋白酶与胰蛋白酶联合酶解法，总加酶量为5‰（w/w）在45℃水解4h后，再加0.2%NaOH碱解2h，蛋白质水解度和氨基酸转化率分别高达80.4%和39.6%。

This paper presented the results of decolouring and enzymatic hydrolysis technology of P.urceolata to prepare a nutritive liquid of composite amino acids.The results showed that KMnO4 was a good decolourant for P.urceolata.The optimum hydrolysis conditions were :using combined enzymes (AS1.389 protease and trypsin,5‰ totally) at 45℃ for 4 hours and then treating with 0.2% NaOH for 2 hours.The degree of protein hydrolysis was up to 80.4% ,and the transformation ratio of amino acids was 39.6%.