高效液相色谱法测定何首乌中大黄酸、大黄素、大黄素甲醚的含量

Determination of Emodin Acid, Emodin and Emodin Methyl-ether in Polygonum multiforum by HPLC

何首乌用70%甲醇超声提取浓缩后，用氯仿萃取分离出游离和结合型蒽醌，得到定量用试液。在439nm检测波长下，以甲醇:异丙醇:水=80:10:10（磷酸调pH值为3.0）为流动相，C18填料为固定相，采用HPLC法测定何首乌中蒽醌类成分含量。平均加样回收率大黄素为100.7%，RSD=1.04.%大黄素甲醚为98.7%，RSD=1.77%% 重复性实验的RSD分别为：大黄酸1.18%，大黄素0.79%，大黄素甲醚0.75%（n=10）。

Using 70% methanol, the Polygonum multiforum extract was concentrated by ultrasonic method .Then concentrated extract was leached with chloroform and separated into free and bound anthraquinones.The mobile phase was V(CH3OH):V(C3H7OH):V(H2O)=80:10:10 (control pH to 3.0 with phosphorus acid ).The HPLC system was consisted of Hypersil BDS C 18(5μm,250nm×46nm) and UV detector set at 439nm.The experiment results show that the average recovery of Emodin was 100.7% (RSD=1.77),and that of Emodin methylether was 98.7% (RSD=1.77%) .The relative standard deviation for Emodin acid ,Emodin and Emodin methylether were 1.18%,0.79% and 0.75% respectively.