^(99)Tc^m(CO)_3-BPHRGD的制备及正常鼠体内生物分布

Preparation of ^(99)Tc^m(CO)_3-BPHRGD and Biodistribution Evaluation in Normal Mice

制备了99 Tc m(CO)3-BPHRGD,并进行体内外生物学评价。在pH=7、75℃条件下反应30min,99 Tc m(CO)3-BPHRGD的标记率均大于80%,纯化后标记物的放射化学纯度大于98%;体外稳定性实验显示,在37℃时,标记物在生理盐水、胎牛血清及半胱氨酸溶液中具有很好的稳定性;正常小鼠体内生物分布数据显示,99 Tc m(CO)3-BPHRGD在血液中清除较快,主要通过肝肾代谢。

^99Tc^m （CO）3-BPHRGD was prepared, and the in vitro and in vivo biological evalu- ations were completed. The labeling yield of ^99Tc^m （CO）3-BPHRGD is more than 80% under optimal conditions（pH=7, reacting at 75℃ for 30 min）, and the radiochemical purity is more than 98 % after purified. Stability in vitro experiments show that the radiolabeled compound shows good stability in physiological saline, fetal bovine serum, and cysteine solution under 37℃. The biodistribution of ^99Tc^m（CO）3-BPHRGD in normal mice shows that the metabolic pathways are mainly though