番茄青枯病植物疫苗胶悬剂保存特性的研究

Study on Storage Characteristics of Colloidal Suspension Agent Used us Plant Vaccine Against Tomato Bacterial wilt Disease

采用50 L全自动发酵罐进行植物疫苗生产菌株FJA T-1458发酵液制备，将其制成琼脂终浓度为2‰的胶悬剂，研究pH、氯化钠浓度对植物疫苗胶悬剂保存特性的影响，并跟踪检测植物疫苗胶悬剂保存过程致病性及防效的变化。结果表明，植物疫苗胶悬剂保存的最适盐浓度为2％～3％，最适pH值为6～8。植物疫苗胶悬剂保存过程中，菌株对番茄青枯病的防效稳定不变，菌体的致病性指标-弱化指数变化小，从生物学特性可视为变化无差异。

The fermentation of Ralstonia solanacearum FJAT-1458 used for production of plant vaccine was conducted in 50 L automatic fermentation container ,and the fermentation broth was prepared to produce with 2‰agar to form a plant vaccine .During plant vaccine colloidal suspension agent storage process ,the characteristics of strain FJAT-1458 was detected under different pH values and different percentages of salt ,and the control efficiency and attenuation index of strain FJAT-1458 were also detected . The results showed that the best storage condition for plant vaccine colloidal suspension agent was pH 6 -8 and 2% -3% of salt. The Control efficiency of plant vaccine colloidal suspension agent against tomato bacteria wilt during its storage was stable and there was no significant difference between the attenuation in biological view.