摘要
选用香菇的杂交菌株农１与野生株Ｑ进行正反双单杂交，得到６个杂交后代。结果表 明：３个正交菌株与３个反交菌株在酯酶同工酶与ＤＮＡ水平上具有极高的相似性，而对杀菌 剂和温度的敏感性显示了明显的遗传差异，且农艺性状遗传差异也十分显著。正反杂交菌株在 核基因相同情况下的遗传差异应主要归于细胞质差异。本研究表明，香菇双单杂交后代是同质 异核体。
The positive and negative di-mon mating of a crossed strain Nongl and a wild strain Q from Lentinula edodes were carried out and six hybrids were obtained. The results show that three strains of the positive cross and three strains of the negative cross exist high similarity on the esterase isoenzyme and DNA molecular level. However, they show distinct genetic differences on sensitivity to prochlorac (germicide)and temperature.The agronomic characters of the hybrids are also evidently diffrenet. Clearly, the genetic differences of hybrids of the positive and negative di-mon mating should be attributed to the differences of cytoplasm. This study indicate that the positive and negative di-mon mating hybrids are homogenous heterokaryons in Lentinula edodes.
出处
《菌物系统》
CSCD
北大核心
2001年第1期94-99,共6页
Mycosystema
基金
博士点基金项目
安徽省教育厅科研基金
关键词
香菇
正反双-单杂交
酯酶同工酶
RAPD
敏感性
农艺性状
同质异核体
遗传分析
Lentinula edodes, The positive and negative di-mon mating, Esterase isoenzyme, RAPD, Sensitivity, Agronomic character, Homeonomous heterokaryon.
