血清结核分枝杆菌复苏因子抗体检测方法的建立及其在肺结核诊断中应用价值的评价

The Value of RpfE-Ab of Mycobacterium tuberculosis for the Diagnosis of Pulmonary Tuberculosis

目的建立结核分枝杆菌复苏因子抗体（RpfE—Ab）免疫印迹检测方法，并评价其在肺结核诊断中的应用价值。方法以原核表达结核分枝杆菌复苏因子蛋白作为抗原，应用免疫印迹法检测88例确诊肺结核患者血清和100例健康人血清中RpfE—Ab，并将结果与TSPOT-TB、抗结核抗体（LAM-Ab）结果进行比对。结果1：血清结核分枝杆菌复苏因子抗体检测方法在诊断肺结核中的敏感性为82．9％，特异性为92．0％。2：血清结核分枝杆菌复苏因子抗体检测方法在诊断肺结核中与TSPOT-TB、LAM—Ab检测方法之间无差异（χ2=0.92，P〉0．05，χ2=0．02，P〉0．05）。结论血清结核分枝杆菌复苏因子抗体检测可与TSPOT-TB、LAM—Ab同时作为肺结核辅助诊断方法。

Objective To establish immunoblots technique for detection of serum antibody to Mycobacteriurn tuberculosis Resuscitation-Promoting factor （Rpf） and evaluate its significance in the diagnosis active pulmonary tuberculosis. Method Prokaryotic expression recombinant protein of MycobacteHurn tuberculosis Rpf was performed used as antigen for antibody detection in sera from 88 patients with active pulmonary tuberculosis and 100 health controls. The results of T SPOT-TB and LAM-Ab were compared simultaneously. Results The sensitivity, specificity, positive predictive value and negative predictive value of Rpf- Ab detection were 82.95%. 92.0%. 91.58%. 87.61% ; There were no statistical significance among three tests （RptE-Ab, T SPOT-TB, LAM-Ab）（ χ2=0.92, P 〉 0.05 ;χ2=0.02, P 〉 0.05）. Conclusion Detection of Rpf antibody in serum has higher sensitivity, specificity, the PPV, NPV ,in the diagnosis of pulmonary tuberculosis, it is promising to be a supplementary technique in the diagnosis of pulmonary tuberculosis.