茉莉酸甲酯与真菌诱导物、水杨酸组合对红豆杉细胞中紫杉醇含量的影响

Effects of the combination of methyl jasmonate with salicylic acid or fungal elicitor on the cell suspension of Taxus chinensis

比较了茉莉酸甲酯与真菌诱导物、水杨酸组合对红豆杉细胞几个抗病相关指标（ＰＯＤ、ＣＡＴ活力、Ｈ２Ｏ２含量）及紫杉醇含量的影响，３种信号分子的组合对ＰＯＤ、ＣＡＴ、Ｈ２Ｏ２及紫杉醇含量的影响是不一致的，ＭＪ单独添加，ＭＪ与ＳＡ联合作用以及ＭＪ与Ｆ５联合作用都可使 ＰＯＤ活力增加，且１２ｈ后Ｈ２Ｏ２含量均升高，约在 ４８ｈ达到高峰，为对照的２倍左右，但 ７２ｈ后，ＭＪ单独添加和ＭＪ与ＳＡ联合作用组中Ｈ２Ｏ２含量变化不大，Ｆ５与ＭＪ联合作用则使Ｈ２Ｏ２含量持续比对照高。ＭＪ单独添加使ＣＡＴ酶活在１４４ｈ后才较对照低，Ｆ５、ＳＡ的加入都可使ＣＡＴ酶活下降，ＳＡ的作用更显著。说明三者的诱导途径并不完全一样，以 ＳＡ和 ＭＪ联合添加对紫杉醇合成的促进作用最大，含量达到细胞干重的 ０．０４％。

The effect of methyl jasmonate (MJ)with salicylic acid (SA) or fungal elicitor (F5)on several correlative indexes of disease resistant and taxol content were studied. The effects of different induction combinations were different. All combinations increased peroxidase (POD) activity, H2 O2 content in-creased after 12h and achieved the maximum at 48h in three induction combinations. There aren't marked difference of H2O2 content between the individual addition of MJ and the combination of MJ and SA after 72h. But the combination of MJ and F5 increased H2O2 content of cells continually. All combinations de-creased CAT activity, especially the combination of MJ and SA ,The combination also increased taxol con-tent markedly, taxol content reached 0.04% DW.