超抗原葡萄球菌肠毒素A、B和C1的T细胞抗原HLA表位预测

Prediction of the HLA epitopes of T cells antigen on superantigen of staphylococcal enterotoxin A,B and C1

为预测超抗原葡萄球菌肠毒素 (SE)家族中的SEA、SEB和SEC1的HLAI和HLAⅡ抗原结合表位 ,并对其活化T细胞作用的机理进行探讨 ,根据已发表的SEA、SEB和SEC1基因全序列 ,用T细胞抗原表位预测软件Guo tif 2 0对其进行T细胞抗原表位预测 ,统计与HLAI和HLAⅡ各抗原位点结合的SEA、SEB和SEC1肽段的出现次数。结果显示 ,SEA、SEB和SEC1具有共同的特点 ,即都是主要与HLAⅠ类分子的A3位点和HLAⅡ类分子的DR1位点具有较强的结合。说明SEA、SEB和SEC1与HLAⅠ类分子和HLAⅡ类分子都有很强的结合性。三者在HLAⅠ和HLAⅡ结合位点上具有较强的同源性。

In order to predict the HLA Ⅰ and HLAⅡ epitopes on SEA,SEB and SEC1 of the superantigen staphylococcal enterotoxin(SE) family,and study the mechanism of SE activating T cells,predict the T cell epitopes of SEA,SEB and SEC1 according to their published complete sequences by using Guotif 2.0 T cell epitopes predicting software,with which the frequences were processed by statistics,to compare with the frequences occurred in the three types of peptide binding to motifs of HLA class Ⅰ and Ⅱ molecules.The results showed all three binding mainly to A3 motif of class Ⅰ molecule and DRⅠ motif of classⅡ molecule.There are in high degree of homogenecity among the three binded epitopes on HLA class Ⅰ and Ⅱ molecules.This study is a fundamental work for the experimental approach on the mechamism of SE activating T cells.