高效液相色谱仪串联三重四级杆质谱法快速测定姜茶中的姜黄素

Determination of curcumin in ginger tea by liquid chromatography mass spectrometry with ultrasonic extraction

建立了高效液相色谱串联三重四级杆质谱法（LC -MS/MS）测定姜茶中的姜黄素的方法。对样品的前处理方法及LC -MS/MS分析条件进行了优化，样品以甲醇为提取溶剂，超声提取，LC -MS/MS多反应监测模式（M RM ）进行测定。姜黄素在0.005～0.1μg/mL 范围内均呈良好的线性关系，相关系数为0.999；检出限为1.0μg/kg。标准加标回收试验，姜黄素添加浓度分别为5.0，10.0，20.0μg/kg ，平均回收率为92.5％～102.5％，相对标准偏差（RSD ）2.0％～2.5％。方法简便、快速、溶剂用量少，可消除姜油带来的干扰，结果准确可靠，适合进行批量样品的测定。

A sensitive method was developed for the determination of curcumin in ginger tea by liquid chromatography mass spectrometry with ultrasonic extraction .In this paper ,the conditions of sample pre-treatment and LC-MS/MS were investigated and optimized .The samples were extracted with methanol . The detection was carried out by LC-MS/MS under the multiple reaction monitoring mode（MRM） .Good linear relationships were obtained in the concentration range of 0 .005-0 .1μg/mL of curcumin ,with corre-lation coefficients of 0 .999 .The limits of detections （S/N=3） of curcumin was 1 .0μg/kg .Under optimized conditions ,the average recoveries with sample spiked the standards at 5 .0 ,10 .0 ,20 .0μg/kg were 92.5%-102.5% ,the RSD were 2 .0%-2 .5% .The method is simple ,rapid and low consumption of solvent ,and the ginger oil disturbance in Ginger Tea samples can be eliminated when MRM mode is adopted .It is fit for the determination of batch samples .