运动训练对SHR/SP大鼠VD模型认知功能及海马ChAT、AchE活性的影响

Effects of exercise training on cognitive function,ChAT and AchE activity in SHR/SP rat vascular dementia model

目的观察运动训练对自发性高血压脑卒中倾向大鼠(SHR/SP)血管性痴呆(VD)模型认知能力及ChAT、AchE活性的影响。方法将雄性SHR/SP大鼠30只,分为假手术组、模型组、运动组,每组各10只,采用分次结扎2-VO法制作VD模型,假手术组、模型组术后正常饲养不做干预;运动组术后采用跑台训练(DSPT-1)8周,训练完成后采用Morris水迷宫检测各组大鼠认知功能,最后处死大鼠取材检测海马胆碱乙酰转移酶(ChAT)及乙酰胆碱酯酶(AchE)活性。结果在定位航行训练中,假手术组大鼠潜伏期明显少于运动组和模型组大鼠,但运动组大鼠潜伏期明显短于模型组大鼠(P<0.05);在空间探索实验中,假手术组大鼠跨越原平台次数明显多于其他两组大鼠,运动组大鼠跨越原平台次数明显多于模型组(P<0.05);运动训练可增加海马ChAT活性及降低AchE活性。结论运动训练可改善SHR/SP大鼠VD模型海马胆碱能系统的功能,进而提高认知能力。

Objective To investigate the effects of the exercise training on the cognitive function, choline acetyltransferase （CHAT） activity and acetylcbolinesterase （AchE） activity in stroke prone spontaneously hypertensive rat （SHR/SP） vascular de- mentia model. Methods 30 male SHR/SP rats were randomly divided into sham operation group,model group and exercise group （n= 10）. The VD model was established by the fractional ligation of bilateral carotid artery（2-VO）. The sham operation group and the model group were given the normal feeding without intervention after operation; the exercise group adopted the treadmill exer- cise（DSPT-1） for 8 weeks. After the exercise, the Morris maze test was conducted for evaluating the cognitive function in each group. The rats were finally killed for detecting the ChAT activity and AchE activity of hippocampus. Results In the positioning navigation training,the latency period of the sham operation group was significantly short than that of the exercise group and the model group, but the latency period of the exercise group was obviously short than that of rats in the model group（P〈0.05） ;in the spatial exploration test, the rats in the sham operation group had more frequency of crossing platform than the other two groups, the exercise group had more frequency of crossing platform in platform quadrant than the model group（P〈0.05） ;the exercise training could increase the ChAT activity and lower the AchE activity of hippocampus. Conclusion The exercise training can improve the function of hippocampal cholinergic system in SHR/SP and then increase the cognitive ability.