高效液相色谱法测定水氯甲痤疮凝胶剂中3组分含量

Content Determination of Three Components in Salicylic Acid Chloramphenicol Metronidazole Acne Gel by HPLC

目的建立测定水氯甲痤疮凝胶剂中3组分含量的高效液相色谱法。方法采用VP—ODSC18色谱柱（150mm×4．6mm，4．6μm），甲醇-水-冰醋酸（50：50：1．2）为流动相，流速为1．0mL／min，检测波长为280nm，柱温为35℃，进样量为20μL，试验过程空调控温在25℃。结果水杨酸质量浓度在60～300μg／mL范围内与峰面积线性关系良好；氯霉素质量浓度在30～150μg／mL范围内与峰面积线性关系良好；甲硝唑质量浓度在15～75μg／mL范围内与峰面积线性关系良好。平均回收率水杨酸、氯霉素和甲硝唑分别为101．65％。100．71％和101．48％，RSD分别为0．78％，1．19％和1．22％（n=6）。结论该方法简单、灵敏、重现性好，可作为水氯甲痤疮凝胶剂中3组分的含量测定方法。

Objective To establish a HPLC method for determining the contents of three components in Salicylic Acid Chloramphenicol Metronidazole Acne Gel. Methods The VP-ODS C18 column（150 mm×4.6 mm, 4.6 μm） was adopted; the mobile phase was methanol-water-glacial acetic acid（50：50：1.2）;the flow rate was 1.0 mL/min;the detection wavelength was set at 280 nm;the column temperature was 35 ℃ ;the injection volume was 20 μL,the air conditioning temperature was at 25 ℃ during the experimental process. Results The linear range of salicylic acid was 60- 300 μg/mL with an average recovery rate of 101.65%. The linear range of ehloramphenicol was 30- 150 μg/mL with an average recovery rate of 100.71%. The linear range of metronidazole was 15-75 μg/mL with an average recovery rate of 101.48% （n =6）, RSD =0. 78%, 1. 19%, 1.22% （n =6）. Conclusion The method is simple,sensitive and better reproducible,can be used as the method for the content determination of three- components in Salicylic Acid Chlorampheni- col Metronidazole Acne Gel.