PC膜微柱阵列制备及其对成骨细胞铺展影响

Fabrication of the Parylene-C Film with Micropillar Arrays and Its Effect on the Spread of the Osteoblast

研究了聚一氯对二甲苯(parylene-C,PC)膜微柱阵列结构的制备及其对MC3T3-E1小鼠成骨细胞生长铺展的影响。在硅基微柱阵列结构表面上沉积聚对二甲苯得到的PC膜微柱阵列结构能促进细胞的黏附和铺展,且柱间距对细胞面积与形态都有显著影响。细胞长度随柱间距的增加而增大;在微柱边长不超过24μm且柱间距小于53.7μm的范围内,细胞铺展面积也随柱间距的增加而增大。

Fabrication of the parylene-C （PC） film with micropillar arrays was researched, and its influences on the growth and spread of the mouse osteoblast MC3T3 - E1 cells were discussed. The PC film with micropillar arrays was achieved through the deposition of the parylene on the silicon based micropillar arrays. The obtained PC film with micropillar arrays can improve the ad- hesion and spread of the cells, and the space between two neighboring micropillars has a great in- fluence on the area and shape of the cells. The cell length increases with the increment of the space between two neighboring micropillars. When the micropillar edge length is less than 24 μm and the space between two neighboring micropillars is less than 53.7μm, the wider the micropil- lar space, the larger the cell spreading area is.