扁枝石松生物碱成分的研究

Studies on Alkaloids of Diphasiastrum complanaturn (L.) Holub

云南西双版纳产扁枝石松(Diphasiastrum complanatum L.)干燥全草用甲醇提取,利用硅胶柱层析、Sephadex LH-20柱层析和反相柱层析进行化学成分分离纯化,从中分离得到6个Lycopodine型生物碱化合物,根据现代波谱技术和理化性质对化合物结构进行鉴定,鉴定结构为:6β-hydroxylycopodine(1),lycopodine(2),6α-hydroxylycopodine(3),4α-hydroxylycopodine(4),lycoclavine(5),15α-methyl-lcopodane-5β,6α-diol(6).以上化合物均为首次从西双版纳产扁枝石松中分离得到.

Six lycopodine alkaloids were obtained by silica gel chromatography, Sephadex LH-20 chromatography and reversed-phase chromatography from the methanol extract of the all plants of Diphasiastrum complanatum （L.） Holub from Xishuangbanna of Yunnan. The chemical structures of these compounds were identified by modern spectroscopic analysis combined with their physico-chemical data. These compounds were identified to be 6β-hydroxylycopodine （1）, lycopodine （2）, 6a-hydroxylycopodine （3）, α-hydroxylycopodine （4）, lycoclavine （5）, 15α-methyl-lcopodane-5β,6α-di- ol （6）. All the six compounds above were isolated from D. complanatum of Xishuangbanna for the first time.