瘢痕中肌动蛋白及肌球蛋白的实验研究

EXPERIMENTAL STUDY OF ACTIN AND MYOSIN IN THE SCAR TISSUE

目的 探讨增生性瘢痕、瘢痕疙瘩及正常皮肤成纤维细胞中肌动蛋白和肌球蛋白 的不同表达情况及其与瘢痕挛缩的关系。方法 取增生性瘢痕 15例 ,瘢痕疙瘩 10例 ,正常皮肤 15例 ,用免疫组织化学方法检测其中肌动蛋白和肌球蛋白 的表达情况 ,同时做细胞培养 ,用流式细胞术检测成纤维细胞中肌动蛋白和肌球蛋白 的表达情况。结果 增生性瘢痕肌球蛋白 的免疫组织化学染色呈阳性 ,而瘢痕疙瘩及正常皮肤肌球蛋白 的表达均为阴性。增生性瘢痕肌球蛋白 的表达较其它两种组织有非常显著性差异 (P<0 .0 1) ,而瘢痕疙瘩和正常皮肤无显著性差异 (P>0 .0 5 )。流式细胞术检测增生性瘢痕、瘢痕疙瘩及正常皮肤三种组织中肌球蛋白 的阳性率分别为 (95 .11± 2 .78) %、(16 .86± 7.11) %及 (5 .31± 1.79) % ,增生性瘢痕肌球蛋白 表达的阳性率较其它两种组织有非常显著性差异 (P<0 .0 1) ,而瘢痕疙瘩和正常皮肤无显著性差异 (P>0 .0 5 )。三种组织中肌动蛋白的免疫组织化学染色均为阳性 ,无显著性差异 (P>0 .0 5 )。流式细胞术检测三种组织中肌动蛋白的阳性率分别为 (77.77±15 .43) %、(88.89± 10 .2 9) %及 (82 .92± 13.48) % ,其表达的阳性率无显著性差异 (P>0 .0 5 )。结论 瘢痕挛缩与肌球蛋白

Objective To investigate the different expression of actin, myosin Ⅱ in hypertrophic scars, keloids and normal skins, and to understand the relationship of actin, myosin Ⅱ and the scar contracture. Methods Fifteen cases with hypertrophic scars, 10 cases with keloids and 15 cases with normal skins were chosen randomly. The expression of actin and myosin Ⅱ were detected by immunohistochemical method in the hypertrophic scars, keloids and normal skins. The fibroblasts isolated from three types of tissue were cultured in vitro, then actin and myosin Ⅱ in three different fibroblasts were measured using flow cytometry. Results The immunohistochemical staining of myosin Ⅱ in hypertrophic scars was positive, while the staining in keloids and normal skins were negative. The positive rate of myosin Ⅱ expression in hypertrophic scars, keloids and normal skins were (95.11±2 78)%, (16.86±7.11)%, and (5.31±1.79)% respectively. There were significant difference between keloids and the two others( P <0.01). The actin expression in three difference tissues were positive, there were no significant difference in hypertrophic scars, keloids and normal skins( P >0.05). The positive rate of actin expression in hypertrophic scars, keoids and normal skins were(77.77±15.43)%, (88.89±10.29)%, and (82.92± 13.48)% respectively, and there were no significant difference( P >0.05). Conclusion Myosin Ⅱ may play an important role in the scar contracture. Actin is the contractile protein of cell, it plays important role in cellular movement. Actin is necessary protein in the cell.