金属螯合固相萃取整体柱的制备及其性能研究

Preparation and Characterization of Polymer Solid Phase Extraction Monolith Immobilized Metal Affinity Ligands

基于金属螯合物中Cu2+与氨基硫醇类化合物(如半胱氨酸的-SH基团)之间的亲和作用,设计合成了一种金属螯合整体柱,并用作固相萃取吸附剂。实验以亚氨基二乙酸(IDA)三齿配体为中间介质,通过化学修饰法键合在聚(甲基丙烯酸缩水甘油酯-乙二醇二甲基丙烯酸酯)(poly(GMA-co-EDMA))整体柱孔表面,再利用配位结合作用螯合Cu2+,合成poly(GMA-co-EDMA-IDA-Cu2+)整体柱。实验以谷胱甘肽为探针测试化合物,考察了该整体柱固定相的萃取性能,并优化了实验条件。在最佳实验条件下,此整体柱对谷胱甘肽的吸附容量为43.15 mg/g,并能有效富集人血浆样品中的氨基硫醇类化合物。

Abstract Based on the affinity interactions between Cu2＋ of metal chelate and-SH groups of aminothiols such as cysteine, a polymer solid phase extraction monolith was designed and synthetized by the tridentate ligand iminodiacetic acid （IDA） immobilized on the surface of poly glycidyl methacrylate-based monolith and after modification with Cu2＋. Glutathione as a model compound was tested to evaluate the performance of the solid phase extraction monolith and optimize enrichment conditions. Under the optimum conditions, the maximum absorption capacity for glutathione of this polymer monolith was determined at 43.15 mg/g, and the polymer monolith could be used for the extraction and enrichment of traee aminothiols in human olasma samoles.