表没食子儿茶素没食子酸酯对大鼠蛛网膜下腔出血后脑血管痉挛影响的实验研究

The research of epigallocatechin gallate on rat cerebral vasospasm after subarachnoid hemorrhage in rats

目的研究表没食子儿茶素没食子酸酯(EGCG)对大鼠蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)的作用并探索其作用机制。方法将24只SD大鼠采用随机数字表法分为4组,即空白组、0.9%氯化钠注射液组、SAH组、SAH+EGCG组,苏木素-伊红(HE)染色观察各组大鼠基底动脉管径的变化,反转录-聚合酶链反应(RTPCR)定量检测各组大鼠血管内皮细胞中血管紧张素Ⅱ(AngⅡ)及内皮素(ET)基因水平。结果 HE染色结果提示SAH组和SAH+EGCG组基底动脉管壁厚度及管腔狭窄程度显著大于空白组、0.9%氯化钠注射液组,而SAH+EGCG组显著<SAH组,差异具有统计学意义(P<0.05);SAH组和SAH+EGCG组内皮细胞AngⅡ及ET的mRNA表达水平显著高于空白组及0.9%氯化钠注射液组(P<0.05),而SAH+EGCG组血管却低于SAH组,差异具有统计学意义(P<0.05)。结论 EGCG能够降低SAH后血管内皮细胞AngⅡ及ET基因水平及其表达,可能具有一定的抗血管痉挛作用。

Objective To study the effect of epigallocatechin gallate（EGCG） on cerebral vasospasm（CVS） after subarachnoid hemorrhage（SAH） and research the mechanism. Methods Twenty-four SD rats were randomly divided into 4 groups, namely control group, saline group, SAH group, SAH＋EGCG group, HE staining of the basilar artery, and RT-PCR were used to detect the expression of angiotensin Ⅱ（AngⅡ） and endothelin（ET） in vascular endothelial cells. Results Basilar artery wall thickening and luminal stenosis in SAH group and SAH＋EGCG group was statistically significant than control, saline group, SAH＋EGCG group was more significant compared with SAH group（P＆lt;0.05）; the expression of AngⅡ and ET′s mRNA in SAH group and SAH＋EGCG group was greater than control and saline group , furthermore, SAH＋EGCG group was statistically less than SAH group （P＆lt;0.05）. Conclusion EGCG can reduce the expression of AngⅡand ET in vascular endothelial cells and attenuate CVS after SAH.