家蚕对2型糖尿病大鼠脂代谢及脂肪细胞因子的影响

Effects of silkworm on lipid metabolism and serum adipose cytokine levels in rats with type 2 diabetes mellitus

目的观察家蚕对2型糖尿病大鼠脂代谢及脂肪细胞因子的影响,探讨其对治疗糖尿病的作用机制。方法以高热量饮食喂养SD大鼠6周加小剂量链脲佐菌素(STZ)建立2型糖尿病大鼠模型,采用随机数字表法分为模型对照组,吡格列酮组,家蚕高、中、低剂量组,共5组,测定其空腹血糖(FBG)、空腹血胰岛素(FINS)、肿瘤坏死因子(TNF)-α、游离脂肪酸(FFA)、脂联素(APN)水平。结果家蚕中、低剂量组及吡格列酮组FBG均有不同程度的降低,与模型对照组差异有统计学意义(P<0.01)。家蚕各剂量组和吡格列酮组大鼠FINS、TNF-α、FFA均有不同程度的下降,与模型对照组大鼠相比差异有统计学意义(P<0.05或P<0.01)。家蚕中、低剂量组和吡格列酮组脂联素与模型组相比显著升高(P<0.01)。结论家蚕可通过调节2型糖尿病大鼠脂代谢及脂肪细胞因子水平,改善其胰岛素抵抗状态,为家蚕的临床应用提供了实验依据。

Objective To determine the effects of silkworm on lipid metabolism and serum adipose cytokine levels in diabetic rats with insulin resistance and to explore the mechanisms for these therapeutic effects. Methods The type 2 diabetes diabetic rat models were fed with high-sugar-fat-diet and low-dose streptozocin （STZ） for 6 weeks, which entailed randomization to model control group, pioglitazone treatment group, high-dose silkworm treatment group, medium-dose silkworm treatment group and low-dose silkworm treatment group, respectively. The levels of fast- ing blood glucose （FBG）, fasting insulin （FINS）, tumor necrosis factor-α （TNF-α）, free fatty acid （FFA） and adiponectin （APN） were assayed. Results Compared with model group, treatment with medium- and low-dose silkworm and pi- oglitazone resulted in FBG reduction to various degrees （all P〈0.01）. All groups but model group yielded a marked de- crease in FINS, TNF-α and FFA （all P〈O.05）. The low- and medium-dose silkworm group and pioglitazone group were associated with a significantly higher level of adiponectin compared with model group （both P〈O.O1）. Conclusion Silkworm alleviates insulin resistance in type 2 diabetic rat models via modulating lipid metabolism and serum adi- pose cytokine levels, thus offering an experimental basis for the clinical application of silkworms.