细菌性阴道病患者治疗前后阴道灌洗液中干扰素-γ和白细胞介素-4表达的研究

Changes in interferon-γ and interleukin-4 of the vaginal lavage fluid in patients with bacterial vaginosis before and after treatments

目的检测细菌性阴道病(BV)患者治疗前后阴道灌洗液中干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)的水平,了解阴道局部免疫状态与治疗转归关系。方法应用酶联免疫吸附法测定健康妇女33例(对照组)、BV患者32例(BV组)治疗前后阴道灌洗液IFN-γ、IL-4水平。结果①与对照组相比,BV组IFN-γ显著下降,差异有统计学意义(P<0.05);而IL-4升高,但差异无统计学意义(P>0.05)。②BV组治疗转归变化,趋向治愈组中IFN-γ因子水平升高,IL-4因子水平显著下降,差异有统计学意义。结论①BV时阴道灌洗液IFN-γ下降,提示IFN-γ降低与阴道局部细胞免疫功能异常密切相关;②Th1/Th2的漂移在下生殖道感染的发病机制中占有重要地位,联合检测阴道局部细胞因子变化,将为指导BV患者的治疗提供合理的依据。

Objective To investigate the levels of IFN gamma （IFN-γ） and interleukine-4 （IL-4） of the vaginal lavage fluid in women with bacterial vaginosis （BV） before and after treatments, and to examine the relation between vaginal local immunity and the prognosis. Methods Enzyme-linked immunosorbent assay was employed to determine the levels of IFN-α and IL-4 of the vaginal lavage fluid collected from 33 healthy women （control group） and 37 wom- en with BV （BV group）. Results Compared to the control group, IFN-γywas significantly decreased （P〈0.05） and IL-4 increased （P〉0.05） in BV group. Following treatment, women BV group who were nearly cured was characterized by increased IFN-γand decreased IL-4 （both P〈0.05）, respectively. Conclusion The reduction in IFN-γin the vaginal lavage fluid is closely related to abnormal vaginal local immunity. Altered levels of Th1/Th2 ratio plays an important role in lower genital tract infection, which when based on the combination of IFN-γ and IL-4, offer rationales for fu- ture treatment of BV.