观音竹蛋白激酶基因BmPti1启动子的克隆与序列分析

Cloning and sequence analysis of the protein kinase gene BmPti1 promoter of Bambusa multiplex var. riviereorum

以观音竹(Bambusa multiplex var.riviereorum)基因组DNA为模板,通过PCR扩增获得蛋白激酶基因BmPti1的启动子片段,长度为1 466 bp,通过PlantCARE启动子预测工具分析表明:该序列中除含有TATA-box、CAAT-box等基本顺式作用元件外,还发现1个参与生理周期调控的元件、1个厌氧诱导的顺式元件,1个干旱诱导的MYB结合位点、1个光诱导MYB位点、1个水杨酸响应元件、1个赤霉素响应元件、1个低温响应元件、2个ABA与VP1响应元件和2个激素响应元件.表明BmPti1基因的表达可能受干旱、光照、氧气、低温等环境因子以及脱落酸、水杨酸、赤霉素等激素的调控.此外,推测BmPti1基因的表达可能与植株的生理周期相关.

A promoter fragment of protein kinase gene DNA template, 1 466 bp in length. Sequence analysis BmPtil was obtained by PCR using genomic showed that the promoter contained the basic cis-acting elements of TATA-box and CAAT-box, a regulation element to participate in menstrual cy- cles, an anaerobic-induced cis-acting element, a drought-induced MYB binding site, a light induced MYB-bit, an ABA response element, a salicylic acid response element, a gibberellin response ele- ment, a low temperature responsive element, two of ABA and VP1 response element and two hormone response elements. That BmPtil gene expression may be regulated by environmental factors such as drought, light, oxygen, low temperature and abscisic acid, salicylic acid, gibberellin and other hormones. In addition, BmPtil gene expression may be related to the physiological cycle of plants.