摘要
目的 :定量测定黄连配伍肉桂前后肉桂酸含量的变化。方法 :色谱柱YWGC18(4 .6mm× 2 0 0mm ,10μm) ,检测波长 2 80nm ,柱温室温 ,流动相乙腈 0 .0 1%磷酸 (2 7∶73) ,流速 1ml·min-1。结果 :肉桂酸与其他组分具有良好的分离度 ,线性范围为 0 .112～ 4.48μg ,加样回收率为 10 0 .0 % ,RSD =0 .6 6 %。 结论 :黄连与肉桂配伍后肉桂酸含量下降 ,且下降程度与黄连比例有关 ;本法可用于含肉桂复方中肉桂酸的含量测定。
Objective:Studying changes of chemical constituents of Cinnamomum cassia after compatibility in Coptis chinensis ;determining the amount of cinnamic acid in the decoction.Method:An YWG C 18 column was used with a mobile phase of CH 3CN 0.01%H 3PO 4(27∶73),the flow rate was 1.0 ml·min -1 and the detection wavelength was 280 nm.Result and Conclusion:The calibration curve of cinnamic acid had good linear relationship in the range of 0.112～4.48 μg.The average recovery was 100.0%, the relative standard deviation was 0.66%. The amount of cinnamic acid was decreased in the decoction, and the decreased degree was related with the ratio of C. chinensis to C. cassia . [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2001年第1期47-49,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金!资助项目 (3990 0 191)
