应用螨体抗原片间接荧光抗体试验和印迹酶联免疫吸附试验诊断肺螨病的研究

Experimental study on the diagnosis of pulmonary acariasis with Map-IFAT and Dot-ELISA.

目的:探讨螨体抗原片间接荧光抗体试验(MapIFAT)和印迹酶联免疫吸附试验(DotELISA)对肺螨病的诊断价值。方法:应用MapIFAT和DotELISA分别对肺螨病组(32例)、正常对照组(32名)、疥疮组(31例)、蠕形螨组(30例)进行检测并作对比分析。结果:MapIFAT血清抗粉螨抗体的阳性率和DotELISA检测抗粉螨特异性IgG的阳性率:肺螨组分别为91%(29/32)和91%(29/32);正常对照组分别为3%(1/32)和0;疥疮组分别为0和16%(5/31);蠕形螨病组分别为0和0。结论:MapIFAT与DotELISA用于肺螨病的诊断均有较高的特异性、敏感性,且两者结果具有一致性,两种方法联合应用可提高诊断肺螨病的准确性。

Objective:To study the diagnostic values of mite antigen piece-indirect fluorescent antibody test(Map-IFAT)and dot-blot enzyme linked immunoadsordent assay(Dot-ELISA)on pulmonary acariasis.Methods :Special serum antibodies were detected by the two methods and the results were compared in4different groups:pulmonary acariasis group (n=32),normal control group (n=32),scabies group (n=31)and demodicidosis group (n=30).Re sults:The positive rates of pulmonary acariasis were91%(29/32)and91%(29/32).The corresponding positive rates were3%(1/32)and0in the control group,0and16%(5/31)in the scabies group,0and0in the demodicidosis group,respectively.Conclusion:Both methods of Map-IFAT and Dot-ELISA have higher specificity and sensitivity,and no significant variability is found between the two test results.The combination of the two methods may improve the correctness diagnosis of pulmonary acariasis.