甲基丙烯酸环氧丙酯诱导细胞转化的部分相关cDNA的克隆

Cloning of cNDAs involved in malignant transformation induced by glycidyl methacrylate

在甲基丙烯酸环氧丙酯 (GMA)体外诱导建立人胚肺成纤维细胞 (HEL F)恶性转化模型的基础上 ,采用 m RNA差异显示 PCR(DD- PCR)技术 ,对恶性转化细胞与正常细胞之间基因的差异表达进行比较分析。结果显示 ,两种细胞在基因表达方面存在明显差异。已克隆到的 3个差异表达的基因片段分别与人乳腺癌转录因子 (ZABC1)、Tbx3和小鼠 E2基因高度同源 ,并在转化细胞中均呈明显的上调表达。结果提示 ,包括 ZABC1、Tbx3和 E2在内的多个基因的激活与失活可能与

The malignant transformation of human embryonic lung fibroblasts(HELF)induced by glycidyl methacrylate(GMA) in vitro was used as a model for comparing gene expression between the transformed cells and controls.Using mRNA differential display PCR(DD PCR) technique, the three cDNA fragments up regulated in the transformed cells were identified and highly homologous to human breast cancer transcription factor(ZABC1),Tbx3 and mouse ubiquitin conjugating enzyme(E2) gene.These results suggest that activation or inactivation of genes including ZABC1,Tbx3 and E2 may be involved in GMA induced transformation.