高温与香烟对大鼠胚胎神经系统发育的影响

Effects of high temperature and cigarette smoke on the development of nervous system in early rat embryos

为探讨高温、香烟及其联合作用对大鼠胚胎神经系统发育的影响 ,用高温、香烟及其联合作用处理孕 8～ 11天的大鼠 ,孕 2 0天后按致畸实验方法观察胚胎神经系统的发育 (包括头长、神经管末闭、体位异常和脑部形态异常 )。结果表明头长、神经管未闭、体位异常和脑部形态异常发生率均与高温温度、香烟剂量及香烟持续时间呈正比关系 (P<0 .0 5 ) ;并与损害时间发生于胚胎发育的时段有密切关系 ,孕 9～ 10日大鼠神经系统异常发生率明显高于其它时段 (P<0 .0 1)。研究结果还显示高温与香烟联合作用其胚胎神经系统异常发生率明显高于高温或香烟的作用 (P<0 .0 1)。提示高温、香烟及其联合作用明显损害孕 9～ 10日的胚胎神经系统。

In order to explore the effect of high temperature or cigarette smoke and their combined effect on the development of nervous system in early rat embryos,pregnant rats at the 8～11th day of gestation were exposed to high temperature and water soluble substances of cigarette smoke.All indexed of embryonic nervous system development were determined with experimental treat logical methods.The results showed that following the increase of temperature or cigarette smoke exposure all indexes correlated with embryonic nervous system development and morphological differentiation were changed, with an apparent dose effect relationship ( P< 0 01).The changes were related to specific phase of embryonic development. The results showed also that the nervous system development and morphological differentiation induced by both high temperature and cigarette smoke were higher than that by any single factor.