茶色素抑制单核-内皮细胞粘附及其机制研究

Study on the inhibition of tea pigments on the adhesion between monocyte and endothelial cells

为研究茶色素 (TP)对氧化型低密度脂蛋白 (Ox- L DL)损伤血管内皮细胞所致单核细胞粘附的影响 ,应用硫酸铜诱导低密度脂蛋白 (L DL )氧化 ,用细胞粘附实验、流式细胞仪荧光强度测定、细胞 EL ISA等方法分别从细胞、蛋白表达等方面研究茶色素对细胞间粘附分子 (ICAM- 1)和血管细胞间粘附分子 (VCAM-1)表达的影响。结果显示 ,80、40、2 0 mg/ L的 TP抑制 Ox- L DL诱导的单核细胞 -内皮细胞粘附 ,抑制 Ox-L DL 诱导的 ICAM- 1和 VCAM- 1表达并呈浓度依赖性。提示 TP抑制 ICAM- 1和 VCAM- 1的表达是其抑制单核细胞 -内皮细胞粘附及抗动脉粥样硬化的机制之一。

In order to study the effects of tea pigments (TP) on the adhesion between monocyte and endothelial cells induced by oxidized low density lipoprotein(Ox LDL), the fluorescence activated cell sorter (FACS) and the cell ELISA were applied. The results showed that TP could inhibit the adhesion between monocyte and endothelial cells( P <0 05),and the expression of inter cell adhesion molecule(ICAM 1) and vascular cell adhesion molecule (VCAM 1) proteins in a dose dependent manner( P <0 05). The results suggested that the inhibition of TP on ICAM 1 and VCAM 1 expression was one of the mechanisms of its antiadhesion between monocyte and vascular endothelial cells, as well as that of its antiatherosclerosis.