GATA2在白血病患者中的表达及其意义

GATA-2 gene expression in leukemia patients and its significance

目的 探讨白血病患者GATA 2基因的表达及其临床意义。方法 应用RT PCR对各类白血病患者GATA 2的表达进行检测 ,慢性粒细胞白血病 (CML)和急性早幼粒细胞白血病 (APL)同时检测bcr abl和PML RARα的表达。结果 初治 复发的急性髓系白血病 (AML)和急性淋巴细胞白血病(ALL)GATA 2的阳性率分别为 93%和 70 % ,慢性粒细胞白血病慢性期 (CML CP)为 83%。正常人骨髓和外周血未检测到GATA 2。缓解期AML患者GATA 2的表达与初治 复发AML相比 ,差异无显著性 ;移植治疗后患者GATA 2的表达率显著下降 ;移植治疗后的CML患者在bcr abl转阴后仍可检出GATA 2 ,而移植治疗后APL患者PML RARα和GATA 2都为阴性。结论 GATA 2基因转录产物在正常人骨髓和外周血中低表达 ,在各类白血病中高表达。缓解期骨髓中仍有GATA 2的高表达 ,移植治疗后GATA 2表达显著下降。GATA 2高表达反映了患者体内残存的白血病干细胞 ,自体或异基因骨髓移植可以更好地清除白血病干细胞。

Objective To investigate GATA 2 gene expression in leukemia patients and its clinical significance. Methods GATA 2 gene transcripts were detected by reverse transcriptase polymerase chain reaction (RT PCR), and bcr/abl or PML/RAR α transcripts were detected in patients with chronic myeloid leukemia (CML) and acute promyelocytic leukemia (APL), respectively. Results The GATA 2 transcripts were revealed in 93% of acute myeloid leukemia (AML), in 70% of acute lymphoblastic leukemia (ALL), and 83% of CML patients, but not in normal bone marrow and peripheral blood cells. GATA 2 expression in remission patients was similar to newly diagnosed or relapsed patients. In post transplantation patients, GATA 2 expression was significantly decreased. In these cases GATA 2 transcripts could be detected with bcr/abl fusion gene disappeared in 2 of 12 CML, whereas neither GATA 2 nor PML/RARα transcripts could be detected in APL. Conclusion GATA 2 transcripts was highly expressed in leukemia patients and had no change in remission but significantly decreased in post transplantation patients. Detection of GATA 2 expression would give information about the residual leukemia stem cells.