小鼠红白血病细胞(MEL)沿红系分化过程中一种新的VL-30样基因的诱导表达

The induction of high-level expression of a novel VL-30 like gene during the chemical-inducing erythroid differentiation of murine erythroleukemia cells (MEL)

目的 研究在药物诱导小鼠红白血病细胞系EML沿红系分化过程中基因表达的变化。方法 用mRNA差异显示法分析二甲亚砜 (DMSO)和血红素 (Hemin)诱导MEL细胞分化前后基因表达的改变 ,并利用改进的cDNA末端快速扩增 (RACE)技术克隆其中一个差示cDNA片段的旁侧序列 ;以Northern杂交分析检测对应基因的表达改变。结果 在差异显示分析中检测到长 115bp的转录本在诱导后表达增强。经RACE扩增后产物长达 1883bp ,和小鼠BVL 1转座成分的内部序列 (195 5～ 40 0 0nt)区段有很高的同源性。Northern杂交表明MEL细胞中该基因至少有 3个转录本 ,在诱导后表达水平均显著增高。结论 在MEL细胞红系分化过中发现了一种新的VL 30样基因。

Objective To study the gene expression during the chemical inducing erythroid differentiation of murine erythroleukemia cells (MEL). Methods Differential display assay was used to analyze the gene expression before and after the induction differentiation of MEL cells by DMSO and hemin; and then, the flanking sequences of one of the cDNA fragment was amplified by modified rapid amplification of cDNA end(RACE) method, Northern blot analysis was adopted to characterize the expression of this gene. Result The expression of a new transcript similar to VL 30 retrotransposon family increased significantly during the chemical inducing erythroid differentiation in MEL cells. The cloned cDNA is 1883bp, and it is highly homologous to the internal region of murine BVL 1 (1?955 4?000?nt). At least three transcripts in MEL cells were detected by Northern hybridization and all of them increased after the induction. Conclusion A new VL 30 like gene is identified for the first time in MEL cells during chemical inducing erythroid differentiation.