摘要
Leaves and stems of 9 chrysanthemum species were taken as explants and inoculated to 6 differential media and root media to research effects of hor- mone combination and explants on chrysanthemum species. The results indicated that stem of 9 chrysanthemum species and leaf of 6 chrysanthemum species all showed higher regeneration capacity, with regeneration rate in 84%-100% and re- generation coefficient at 2.3-9.4. However, none adventitious buds were found differ- entiated from leaves of ＂28＂ and ＂5-17＂, and leaf regeneration capacity of ＂11＂ was lower, either. Most chrysanthemum species proved higher in stem regeneration ca- pacity, instead of leaf. Therefore, chrysanthemum species can be applied for rapid reproduction and genetic engineering.
以9个菊花品种叶片、茎段为外植体，用6种分化培养基与3种生根培养基直接再生不定芽，研究激素组合及外植体对不同菊花品种直接再生的影响。结果表明，9个品种的茎段与6个品种的叶片都有较高的再生能力，但品种“28”，“5-17”的叶片未分化出不定芽，品种“11”的叶片再生率也不高；多数菊花品种茎段的再生能力高于叶片；再生率为84％～100％，繁殖系数为2．3～9．4，可应用于工厂化快繁与基因工程育种。
