福建省HIV-1病毒基因亚型分布的研究

Genetic Subtyping Analysis of HIV-1 Strains in Fujian Province

目的 了解福建省HIV 1各亚型的流行情况 ,帮助分析其传染来源和判断流行趋势。方法 采集福建省检出的 13例HIV 1感染者的外周血单核细胞 (PBMCs) ,提取核酸后用套式聚合酶链反应技术进行扩增HIV 1膜蛋白 (env)基因C2 V3区目的核酸片段 ,应用异源双链泳动分析法 (HMA)进行HIV 1亚型的分析。结果 13份标本PCR扩增均阳性 ,亚型分析结果表明福建省存在HIV 1B亚型和E亚型。结论 HIV 1B亚型和E亚型可能是福建省流行的主要亚型 ,主要经性途径由境外传入 ,且经性传播途径在人群中流行。异源双链泳动分析法可作为HIV

Objective To investigate the epidemiology of HIV 1 in Fujian Province,in order to identify the source of HIV 1 transmission and analyze the trend of HIV 1 epideic.Methods The nuclear acids were extracted from peripheral blood mononuclear cells(PBMCs) collected from 13 HIV 1 infected individuals found in Fujian,and DNA fragments(the C2 V3)region,about 782bP)of HIV 1 env gene was amplified by nested PCR,the HIV 1 genetic subtypes were then assayed by heteroduplex mobility assay(HMA).Results Show that 13 samples were determined to be positive by PCR amplification,HIV 1 subtype B and E were found in Fujian.Conclusion HIV 1 subtype B and E may be dominant in Fujian Province,subtype B and E were mainly transmitted into Fujian from abroad and spreaded via sexual transmission route among populations.HMA might be a useful screening method for HIV 1 molecular epidemiological analysis.