转化生长因子-α在小鼠Ehrlich腹水癌细胞中的表达

Expression of Transforming Growth Factor-α in Mouse Ehrltch Ascites Carcinoma Cells

本研究应用cDNA寡聚核苷酸探针作分子杂交以及放射免疫测定,首次证明了小鼠Ehrlieh腹水癌细胞中有TGFα—mRNA和TGFα—免疫活性分子表达,尤以晚期癌鼠的癌细胞表达更强。这可能是由于自分泌机制的正性反馈环路作用所致,是小鼠Ehrlich腹水癌细胞快速增殖的重要原因之一。

Transforming growth factor-α (TGF-α) is a single chain polypeptide consisted of fifty amino acid residues Expession of the TGF-α gene has been demonstrated in a variety of tumor,especially in parenchymal tumor cells but not in hematopoietic tumor and Ehrlich ascites carcinoma cell lines. We investigated the expression of TGF-α in mouse Ehrlich ascites carcinoma cells from 5 th and 12th day after inoculation.TGF-α was detected by radioimmunoassay and the TGF-α-mRNA was identified with spot hybridization method. The results show that there was expression of both TGF-α immunoactive molecule and TGF-α-mRNA in mouse Ehrlich ascites carcinoma cells.The intensity of expression in mice after inoculation 12 day was more than that after 5 day.