西沙比利通过一氧化氮影响大鼠胃HCO_3^-分泌的实验研究

Experimental Study of the Effect of Cisapride on the Secretory of HCO_3^- in Rat's Gastric and its Relation with Nitric Oxide

本文研究了西沙比利对大鼠胃粘膜损伤的影响和对胃粘膜—氧化氮含量分泌的影响 ,结果表明 :加入无水乙醇后 ,西沙比利 1mg/kg组胃粘膜溃疡面积及溃疡深度显著低于空白对照组 ( 10 .2± 6.9mm2 vs 2 6.1± 13.5mm2 )( 18%± 3%vs 5 0 %± 2 7% ) (P <0 .0 5 ) ,西沙比利 0 .5mg/kg组溃疡深度 ( 39%± 11% )和 2mg/kg组溃疡深度 ( 18%±7% )亦显著低于空白对照组 (P <0 .0 5 )。给予西沙比利 1mg/kg后 ,其大鼠一氧化氮 ( 2 3.32± 7.40 μmol/mg)显著高于空白对照组 ( 16.93± 3.87μmol/mg) (P <0 .0 5 )。加入西沙比利 2mg/kg后 ,大鼠胃粘膜一氧化氮含量 ( 4.35± 1.5 2μmol/mg)显著低于空白对照组 (P <0 .0 5 )。西沙比利 0 .5mg/kg组一氧化氮含量与空白对照组间差异无显著意义( 16.76± 1.0 6μmol/mg) (P >0 .0 5 )。西沙比利 1mg/kg组胃HCO3-分泌率显著升高 ( 2 0 2 %± 60 %vs 119%± 40 % )。空白对照组和西沙比利 0 .5mg/kg组无此变化。西沙比利 1mg/kg组加入无水乙醇 30分钟后HCO3-恢复率为 810 %± 2 2 0 % ,与加入无水乙醇 15分钟比较 ( 12 0 0 %± 40 0 % )差异有显著意义 (P <0 .0 5 )。西沙比利 2mg/kg组HCO3-分泌率显著降低 ( 68%± 37%vs 12 7%± 2 7% ) (P <0 .0 5 ) ,加入无水乙醇

This study was performed to study the mechanism of cisapride on gastric mucosa protection. Method: Rats were divided into control group (distilled water was infused orally), low dosage group (cisapride 0.5 mg/kg was infused orally), medium dosage group (cisapride 1 mg/kg was infused orally) and high dosage group (cisapride 2 mg/kg was infused orally). After infued alcohol 1.5 ml orally, the area of gastric ulcer, volume of nitric oxide in gastric mucosa and the output of HCO 3 - in gastric mucosa was measured. Result:The area of ulcer in 1 mg/kg group is significently lower than control group (10.2±6.9 mm 2 vs 26.1±13.5 mm 2, P<0.05). The depth of ulcer in three cisapride groups are all significently lower than control group. The volume of nitric oxide in 1 mg/kg group is significently higher than control group (23.32±7.4 μmol/mg vs 16.93±3.87 μmol/mg, P<0.05). While in 2 mg/kg group, it's significently lower than control group(4.35±1.52 μmol/mg). The output of HCO 3 - in 1 mg/kg group is higher than control group. Conclusion: Cisapride has protective effection on gastric mucosa. Cisapride 1 mg/kg can increase the volume of nitric oxide and the output of HCO 3 -. While cisapride 2 mg/kg can decrease the volume of nitric oxide and its protective effect is thinner than 1 mg/kg.