红花水提物抗自由基保护心肌细胞的作用

Effects of Saffron Aqueous Solution on Auti-freeradical and Protective Heart Cells

目的 :观察自由基对心肌细胞的损伤及红花水提物的保护作用。方法 :培养的细胞分 3组 ,正常组加生理盐水 ,损伤组加黄嘌呤、黄嘌呤氧化酶 ,药物组加红花水提液后再同损伤组处理 ,继续培养 4h后测活细胞代谢噻唑兰的比色值及培养液中 LDH。结果 :LDH:药物组 (95.50± 1 1 .60 )高于正常组 (86.38± 1 3.0 9,P<0 .0 5) ,低于损伤对照组 (30 7.2 9± 46.88,P<0 .0 1 )。比色值 :药物组 (0 .884± 0 .0 88)虽低于正常组(0 .945± 0 .1 0 2 ,P<0 .0 5)但高于损伤组 (0 .1 40± 0 .0 92 ,P<0 .0 1 )。结论 :自由基可致心肌细胞损伤 ,红花水提物可保护心肌细胞。

Objective:To study the damage of free radical and the protection of saffron aqueous solution to heart cells.Methods:Cultured cells were divided into 3 grops.Normal group was added with mormal saline,damage group with xanthine and xanthine oxidase,and drug group with saffron aqueous solution befor treated as damage group.Four hours later,the survival heart cells were tested by colorimetric method and the LDH,release from damage cells,were be tested.Results:LDH activity in saffron group(95 50±11 60)were higher than that of normal control(86 38±13 09, P <0 05)but lower than that of damage group(307 29±46 88, P <0 01).The colorimetric value in saffron group(0 884±0=0 88)were lower than that of normal group(0 945±0 102, P <0 05)and higher than that of damage group(0 140±0 092, P <0 01).Conclusion:Saffron aqueous solution could protect the heart cells.