依脉特灵防治大脑中动脉阻塞性脑梗塞的实验研究

Effect of Isotetrandrine on Cerebral Infarction due to Middle Carebral Artery Occlusion in Rats

将依脉特灵用于大脑中动脉阻塞的Wistar大鼠。分批、定期检查神经症状、大脑缺血细胞面数密度,测量大脑梗死面积,并分别与未给依脉特灵的大脑中动脉阻塞组比较。发现:术前预防治疗组,术后10min,15min.20min大脑缺血细胞面数密度显著减少;术后治疗组,术后各时间神经症状均减轻,术后24h、3天、7天大脑梗死面积百分比显著降低。表明依脉特灵对大鼠大脑中动脉阻塞性脑梗塞有一定的防治作用。

In order to investigate the effect of IT on infarction of the brain, IT (3.3mg/kg) was infused intraperitoneally in rats before and after middle cerebral artery occlusion (MCAO). The density of ischemic cells in the somatosensory area of the frontoparietal cortex in the rats treated with IT killed 10min, 15min and 20min after MCAO was 2.3±1.1, 2.0±0.4. 17.0±1.7 respectively, being significantly less than the rats without IT treatment (7.8±1.4, 11.5±1.7, 34.0±1.4, P<0.05); the averaged size of brain infarction in the rats treated with IT killed at the 24th h, and on the 3rd and 7th day after MCAO was 14.1±2.1, 8.6±0.6. 6.2±1.5 respectively, being smaller than in the control rats without IT administration (26.4±2.5, 18.9±2.4, 15.5±1.7, P<0.05); the neurologic deficits were less severe. The results show that IT exhibits a preventive and therapeutic effect on infarction of the brain.