1型糖尿病诊断初期某些细胞因子的改变

The changes of some cytokines in the newly-diagnosed patients of type 1 diabetes mellitus

目的 探讨细胞因子 (CK)在 1型糖尿病中的作用 ,了解其与糖尿病 HL A易感基因及糖代谢的关系。方法 放免法或酶免法检测新诊断 1型糖尿病病人血清 6种 CK、胰岛素和 C肽 ;斑点杂交法检测 4种糖尿病 HL A易感基因 ;微柱法测定 Hb A1c。用 SPSS软件处理数据。结果 病人组 IL - 2、IL - 4低于对照组 ,IL - 1β、IFN-γ/ IL - 4、IL - 12 / IL - 4比值 ,都显著高于对照组 ;IFN-γ、IL - 4、IL - 1β分别与 IL - 12正相关 ,IL - 12 / IL - 4与 IFN-γ/ IL - 4正相关 ;空腹胰岛素与 IL - 1β和空腹 C肽与 IL - 12分别呈正相关。结论 新诊断 1型糖尿病病人存在 IL - 4、IL - 2低下及 IL - 1β的分泌增高 ;1型糖尿病病人存在 TH1优势 ;IL - 12与 IL - 4正相关 ,表明了它们对 TH1、TH2细胞的调节关系 ;CK与 Hb A1c无显著相关性 ;HL A-DQB1* 0 2 0 1携带者 TNF-α较高。

Objective To find out the role of cytokines (CK) in the pathogenesis of type 1 diabetes and the relationship between CK and metabolism and inheritance.Methods We measured the level of six cytokines,C peptide,insulin with RIA or ELISA,defined HLA gene types with blot hybridization of type 1 diabetes patients and determined HbA1c of newly diagnosed diabetes patients.SPSS soft package was used in data analysis.Results IL 2 and IL 4 were lower,and IL 1β,the ratios of IFN γ/IL 4 and IL 12/IL 4 were higher significantly in the patient group than that in the control group.IL 12 correlated positively with IFN γ,IL 4,and IL 1β respectively.There was positive correlation between the ratio of IL 12/IL 4 and the ratio of IFN γ/IL 4,between fasting insulin level and IL 1β,and between fasting C peptide level and IL 12.Conclusion Newly diagnosed diabetes patients had a defect of IL 4,IL 2 secretion and an elevation of IL 1 β.T H1 bias exited in type 1 diabetic patients.The positive lineal relation between fasting insulin and IL 1 β and between fasting C peptide and IL 12 exited.TNF α elevated in HLA DQB1*0301 carrier compared with non carrier.