昌吉地区奶牛子宫内膜炎主要病原菌分离鉴定与中草药体外抑菌实验

Isolation and identification of main pathogen causing dairy cattle endometritis and antibacterial experiment of Chinese herbal medicine in vitro in Changji Area

导致奶牛子宫内膜炎的主要病菌是环境中的致病菌。为了明确引起昌吉周边地区规模化牛场奶牛子宫内膜炎的主要致病菌,本实验对昌吉地区不同规模的奶牛养殖企业和养殖场无菌采集的15份患有子宫内膜炎的奶牛子宫分泌物进行实验室诊断、主要致病菌的分离鉴定,同时使用琼脂扩散法(K-B法)测定了8种单味中草药水煎剂对分离菌株的抑制作用(抑菌圈大小判定)。筛选出适合于本地区奶牛子宫内膜炎治疗的2个口服中草药复方A和复方B,为该地区奶牛子宫内膜炎的治疗和防治提供了依据。

Pathogenic bacterium in environment is the main cause of dairy cattle endometritis. In order to find out the main pathogenic bacteria around Changji Area, 15 samples of uterus secretion from different dairy cattle farm were collected for laboratory diagnosis. Main pathogens were isolated to identify, and AGAR diffusion method(K-B) was used to test the resistance of isolated strains to 8 kinds of single water decoction of Chinese herbal medicine(size of bacteriostatic circle).The oral Chinese herbal medicine compound agent A and B were selected for dairy cattle endometritis treatment in this area. It could provide beneficial experimental basis for treatment and prevention to dairy cattle endometritis in Changji Area.